NMR spektrometers at UiB
The Department of Chemistry has four operational NMR-spectrometers: Two instruments, the 600 MHz and 850 MHz spectometers are located in the NNP-building, while the two 500 MHz spectrometers are located in Realfagsbygget.
The 850 MHz magnet is the core spectrometer of the NNP (Norwegian NMR platform) project, financed by The Research Council of Norway. Its installation was done by May 2016. It will have a key role to greatly enhance the reach and impact of a range of research areas by giving them an entirely new capability: access to explore much larger molecules than current equipment allows (using the TROSY-effect on proteins in particular). Moreover, research needing to identify low-concentration metabolites or other substances will also gain immensely from the enhanced sensitivity and resolution power of the new instruments.
The instrument is equipped with a 1H/13C/15N TCI triple-resonance CryoProbe. In addition an automatic sample changer (SampleJet) is available for 5x96 tubes (refridgerated, in 5 racks) + 30 positions at room temperature.
The new 600 MHz instrument was installed in November 2017 at the NNP building next to the 850 MHz, and it replaced the aging AV600 which was located in Realfagsbygget. This instrument was financed by Bergen forskningsstiftelse, Sparebankstiftinga Sogn og Fjordane, Helse Bergen, and University of Bergen. The spectrometer has a versetile role, and is used for everything from organic chemistry to metabolomics and structural biology.
The instrument is equipped with a cryogenic probe with four RF channels (1H, 13C, 15N, and 31P). It also has the new generation of electronics (NEO), featuring improved dynamic range, faster control and scalability. The automatic sample changer (SampleJet) has five racks for 96 tubes, where the temperature can be set individual for each rack. In addition, it has room for 96 standard length NMR tubes at room temperature.
The Bruker 500 MHz wide-bore spectrometer is located at Realfagsbygget, in room 3002. This instrument is tailored for solid-state NMR, micro-imaging and diffusion. The following probeheads are available:
- Triple-resonance CP-MAS (Magic Angle Spinning) probehead 1H/13C/15N, and additional RF inserts (1H/31P/23Na-29Si), for both 4 mm and 2.5 mm rotors.
- Double resonance modes: 13C/31P; 15N/13C
- Triple resonance modes: 31P/29Si-65Cu; 13C/15N-2H
- 1H channel on the 4mm MAS probe is tunable to 19F. This option will soon be available also for the 2.5mm MAS probe.
- Triple-resonance flat-coil probehead (1H/13C/15N) with a 31P switch (13C-31P switch), for NMR studies on oriented samples.
- MICWB40 micro-imaging probehead, with selective 1H RF insert, and double-tuned 1H/X RF insert (on several diameters). MICRO2.5 gradient system (2.5 G/cm/A) with amplifiers (X,Y,Z) 2xGREAT -60 (60A). Using ParaVision for multi-dimensional MRI/MRS data acquisition, reconstruction, analysis, and visualization.
- Variable-temperature Diffusion probehead with Z-gradient: BBI 500W2/S4 H&F-BB-D-05 DIFF.
The instrument was puchased by UiB as part of the Norwegin NMR Platform, and it was installed in the autumn of 2015.
The AV500 instrument is located at Realfagsbygget, in room 3062. It is mainly used for short routine 1H and 13C experiments where the main goal is identification and characterization of organic compounds. It is equipped with a Broadband Observe (BBO) probe. The instrument has an automatic sample changer where the users can put their sample in queue using the IconNMR software. Several other probes are available for this instrument:
- Broadband Observe (BBO) probe: BBO (1H, BB) from Bruker Biospin.
- Selective fluorine (SEF) probe: Selective observation of 19F with decoupling of 1H (SEF 19F) from Bruker Biospin.
- Diffusion probe: diff30 from Bruker Biospin, z-gradient (1200 Gauss/cm). Exchangeable rf-coils (1H and 13C).
- Magic Angle Spinning (MAS) probe
The instrument is mainly used in IconNMR mode, but it is also possible to run TopSpin upon request.
The AV600 was discharged in December 2017 and was replaced by the NEO600 that is currently at the NNP building.
In addition, the DPX400 spectrometer is no longer in use. It was discharged on May 20th 2015, and replaced by the AV500 instrument. The DPX400 magnet now belongs to the magnet museum located in the southeast corner of the Chemistry Department, 3rd floor. It was placed next to a 200-MHz magnet that is not longer in use either.