Stem Cell Research
The aim of the research is to better understand mechanisms underlying endocrine disorders in order to optimize personalized diagnosing and treatment (regenerative medicine) of these diseases.
Group leader: Helge Ræder
To achieve our goals we study patient registries and patient-derived stem cells, directing the cells to become model systems for endocrine disorders, for example diabetes or diseases affecting reproductive cells (ovarian cells and testicular cells), adrenal gland cells and other cell types involved in endocrine systems. By doing so, we are able to better understand both the normal development and the mechanisms behind disease progression.
We employ registry studies, cell biology techniques (immunofluorescence), proteomics, transcription analyses (mRNA), genomics and epigenomics, including miRNA profiling, both for individual cells and tissues, in addition to bioinformatics.
Thus far, we have established and published results from studies in human cell models for diabetes based on skin cells from diabetes patients (MODY patients), and we are trying to develop methods for patients with monogenic causes for disorders of sexual development. The research leader also coordinates a multiregional treatment service for patients with disorders of sexual development. We cooperate with researchers in the US and Switzerland.
Read more at our web page here.
- Adrian Teo, Nanyang Technological University, Singapore
- Beate Heissig, Tokyo University, Japan
- Berit Strand, Norwegian University of Science and Technology (NTNU), Norway
- Hanne Scholz, University of Oslo (UiO), Norway
- Marco Metzger, Frauenhofer Institute, Germany
- Pedro Herrera, University of Geneva, Switzerland
- Rohit Kulkarni, Joslin Diabetes Center, Boston, USA
- Legoy TA, Vethe H, Abadpour S, Strand BL, Scholz H, Paulo JA, Raeder H, Ghila L, Chera S: Encapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signalling. Sci Rep 2020;10:414
- Furuyama, K., S. Chera, L. van Gurp, D. Oropeza, L. Ghila, N. Damond, H. Vethe, J. A. Paulo, A. M. Joosten, T. Berney, D. Bosco, C. Dorrell, M. Grompe, Raeder, B. O. Roep, F. Thorel and P. L. Herrera (2019). “Diabetes relief in mice by glucose-sensing insulin-secreting human alpha-cells.” Nature 567(7746): 43-48.
- Ng NHJ, Jasmen JB, Lim CS, Lau HH, Krishnan VG, Kadiwala J, Kulkarni RN, Raeder H, Vallier L, Hoon S, Teo AKK: HNF4A Haploinsufficiency in MODY1 Abrogates Liver and Pancreas Differentiation from Patient-Derived Induced Pluripotent Stem Cells. iScience 2019;16:192-205
- Vethe H, Ghila L, Berle M, Hoareau L, Haaland OA, Scholz H, Paulo JA, Chera S, Raeder H: The Effect of Wnt Pathway Modulators on Human iPSC-Derived Pancreatic Beta Cell Maturation. Front Endocrinol (Lausanne) 2019;10:293
- Bjørlykke Y, Søviknes AM, Hoareau L, Vethe H, Mathisen AF, Chera S, Vaudel M, Ghila L and Ræder H. Reprogrammed cells display distinct proteomic signatures associated with colony morphology variability”. Stem Cells Int 2019;2019:8036035
- Legoy TA, Ghila L, Vethe H, Abadpour S, Mathisen AF, Paulo JA, Scholz H, Raeder H, Chera S: In vivo hyperglycaemia exposure elicits distinct period-dependent effects on human pancreatic progenitor differentiation, conveyed by oxidative stress. Acta Physiol (Oxf) 2019:e13433
- Loo LSW, Vethe H, Soetedjo AAP, Paulo JA, Jasmen J, Jackson N, Bjorlykke Y, Valdez IA, Vaudel M, Barsnes H, Gygi SP, Raeder H, Teo AKK, Kulkarni RN: Dynamic proteome profiling of human pluripotent stem cell-derived pancreatic progenitors. Stem Cells 2019
- The role of the carboxyl ester lipase (CEL) gene in pancreatic disease. Johansson BB, Fjeld K, El Jellas K, Gravdal A, Dalva M, Tjora E, Ræder H, Kulkarni RN, Johansson S, Njølstad PR, Molven A. Pancreatology. 2018 Jan;18(1):12-19 Epub 2017 Dec 5. Review. doi: 10.1016/j.pan.2017.12.001. PMID: 29233499
- Springboard to an academic career-A national medical student research program. Jacobsen GW, Ræder H, Stien MH, Munthe LA, Skogen V. PLoS One. 2018 Apr 30;13(4):e0195527. doi: 10.1371/journal.pone.0195527. PMID: 29708980
- Probing the missing mature β-cell proteomic landscape in differentiating patient iPSC-derived cells. Vethe H, Bjørlykke Y, Ghila LM, Paulo JA, Scholz H, Gygi SP, Chera S, Ræder H. Scientific Reports. 2017 Jul 6;7(1):4780. doi: 10.1038/s41598-017-04979-w. PMID: 28684784
- Anatomy and evolution of database search engines — a central component of mass spectrometry based proteomic workflows. Verheggen K, Raeder H, Berven FS, Martens L, Barsnes H, Vaudel M. Mass Spectrom Rev. 2017 Sep 13. doi: 10.1002/mas.21543
- The role of the carboxyl ester lipase (CEL) gene in pancreatic disease. Johansson BB, Fjeld K, El Jellas K, Gravdal A, Dalva M, Tjora E, Ræder H, Kulkarni RN, Johansson S, Njølstad PR, Molven A. Pancreatology. 2017 Dec 5. pii: S1424-3903(17)30890-6. doi: 10.1016/j.pan.2017.12.001