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Flow Cytometry Core Facility

SONY SH800

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SONY SH800

The SH800 is a bench top high-speed multilaser flow cytometer and cell sorter, designed to be affordable and easy to use. 

The cell sorter has a total of 8 parameters, including forward and back scatter, and 6 fluorescent parameters. The cell sorter also has an extra filter set especially for detecting fluorescent proteins. 

 

Specifications

Cell sorter and analyser

488nm (blue) laser

561nm (yellow-green) laser

 

Six fluorescent emission detectors (with examples of fluorochromes/dyes):

FL1 (525/50) – eGFP, CFSE, FITC, Alexa Fluor 488, eYFP

FL2 (585/30) - PE

FL3 (617/30) – (488nm) PE-Texas Red, PI & (561) DsRed, tdTomato, mCherry

FL4 (665/30) – (488) 7-AAD, PE-Cy5, PerCP & (561) mPlum

FL5 (720/60) – PE-Cy5.5, PerCp-Cy5.5

FL6 (785/60) – PE-Cy7

 

Extra optical filter setup for Fluorescent proteins (GFP, RFP, YFP)

535/30,  510/20, 785/60, 585/40 and 640/30

 

Due to the SH800s automatic setup and its easy to use features, the SH800 is available for individual training and use.

The first 5 uses are supervised to ensure proper training.