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Flow Cytometry Core Facility

Hyperion - Image mass cytometry

The HyperionTM Imaging System is a high-parameter imaging system capable of analyzing 4 to 37 protein markers at subcellular resolution in fixed tissue sections or cell smears. With the ability to utilize up to 135 channels to detect additional parameters, the Hyperion Imaging System is ideal to meet researcher needs today and well into the future.

The Hyperion Imaging System is based on the HeliosTM mass cytometry platform. This high-dimensional imaging platform uses mass-tagged antibodies to markers alongside cell structural features in tissue and cell smears.

Mass-tagging involves separation of signals based on the differences in mass, resulting in distinct signals for each marker without the need for compensation associated with fluorometric techniques yet far more specific and sensitive than tag-free techniques.

The metal tags can be defined within 1 Da spatial resolution in tissue and cell smears, resulting in a unique spatial and parametric definition of the cells in situ. The system enables understanding of protein behavior and interactions to drive biological breakthroughs.

Hyperion Imaging System

Channels135
Mass range75–209 amu
Abundance sensitivity0.3% for 159Tb
Frequency200 pixels/sec
Detection limit≥400 copies per μm2
Dynamic range4 orders of magnitude
CalibrationAutomated
Operating systemWindows® 7 Pro 64-bit
Data storage>6 TB RAID (mirrored)
Switch time (between modes)12 hr
Cross-cell contamination (redeposition)≤2% (selected direction)
Crosstalk pixel to pixel≤15% at 200 pixels/sec
Wet tissue thickness for full ablation≤7 μm
Addressable sample size on slide≥15 mm x 45 mm
Optical view of sample≥ 250 μm x 250 μm
File typeTXT, multipage TIFF, OME-TIFF, MCD
Scan area≥1 mm2/2 hr (@200 Hz)

 

 

https://www.fluidigm.com/products/hyperion-imaging-system#specifications

 

The Hyperion image mass cytometer was funded by the Faculty of Medicine, the Haukeland University Hospital and the Center for Cancer biomarkers (CCBIO). 

The Hyperion is located on 5th floor of the Laboratory Building at UiB. Access and management of this platform is through the Flow Cytometry Core Facility