Laser microdissection system
CCBIO has access, through our mother department the Department of Clinical Medicine, a new Laser Microdissection System which is available in our research environment. This enables the user to isolate areas of tissue, or even single cells, from FFPE (formalin-fixed, paraffin-embedded) or frozen tissue sections. The LMD7 comes with a high-quality Leica microscope and camera, and allows for either brightfield or fluorescent imaging.
Laser-microdissected tissue is very suitable for downstream genomic, transcriptomic, proteomic or metabolomic analyses.
• High-quality Leica microscope
• High powered laser (cuts through almost “everything”)
• Gravity-assisted tissue isolation
• Four air-objectives with 5x, 10x, 40x and 63x magnification
• Fluorescence filters (RGB and Cy5)
• Several different collection alternatives (tube caps, 96-well plates, 8/12-tube strips)
• User-friendly software
The Leica LMD7 is located at the Department of Pathology, 2nd floor, Sentralblokken, Haukeland University Hospital, room 5322 (AV-room). The LMD7 is owned by Department of Clinical Medicine (K1). The following have contributed to the purchase: Faculty of Medicine, Pancreas Research Group (Anders Molven), Tumor Biology Research Group (Lars A. Akslen), Neuromics lab (Charalampos Tzoulis), Renal Research Group (Hans-Peter Marti). For booking, go to the MIC booking system. First-time users are required to book a training session. Low cost for use (50 NOK/h), plus minimum 2 hours of supervised use (450 NOK/h) for new users. Superusers/instructors are Kenneth Finne, Tariq Osman and Irene Flønes.