Pål Puntervoll's picture

Pål Puntervoll

NORCE, Uni Research Miljø - Anvendt bioteknologi
  • E-mailPal.Puntervoll@uni.no
  • Phone+47 55 58 40 78+47 412 85 142
  • Visitor Address
    Høyteknologisenteret i Bergen, Bioblokken
    Thormøhlensgate 55, 5. etg
    5008 Bergen
  • Postal Address
    Postboks 7800
    5020 Bergen

ETECvac: Development of a vaccine for enterotoxigenic E. coli based on the heat-stable toxin

Enterotoxigenic E. coli (ETEC) expressing the heat-stable toxin (ST) is among the most important causes of diarrheal disease in children less than 5 years of age in low- and middle-income countries. Diarrhea causes ill health and malnutrition, and is the second most common cause of death among children. Currently available prototype ETEC vaccines target colonization factors and the heat-labile toxin, but not ST. They have so far failed to confer adequate protection, and development of an ST-based vaccine has a high priority.

The ETECvac project is based on a unique and comprehensive ST mutational library, established at the University of Bergen (UiB) and Uni Research, which allows an unprecedented detailed study of the toxin's properties. The main aims of the ETECvac project are to design safe and protective ST vaccine candidates, and to establish the first human challenge model for estimating the protective efficacy of an ST vaccine.

The research will be conducted at UiB and Uni Research, in close collaboration with partners at Institut Pasteur, Paris, France, and Indian Institute of Science, Bangalore, India.

The project is funded by the Research Concil of Norway through the GLOBVAC programme.

Recent publications

  • Diaz, Yuleima; Govasli Larsen, Morten Andreas; Zegeye, Ephrem Debebe; Sommerfelt, Halvor; Steinsland, Hans; Puntervoll, Pål. 2019. Immunizations with enterotoxigenic Escherichia coli heat-stable toxin conjugates engender toxin-neutralizing antibodies in mice that also cross-react with guanylin and uroguanylin. Infection and Immunity. 87e:00099-19: 1-12. doi: 10.1128/IAI.00099-19
  • Bjerga, Gro Elin Kjæreng; Larsen, Øivind; ARSIN, Hasan; Williamson, Adele Kim; Garcia-Moyano, Antonio; Leiros, Ingar; Puntervoll, Pål. 2018. Mutational analysis of the pro-peptide of a marine intracellular subtilisin protease supports its role in inhibition. Proteins: Structure, Function, and Bioinformatics. 1-13. doi: 10.1002/prot.25528
  • Eide, Marta; Rydbeck, Halfdan; Tørresen, Ole K.; Lille-Langøy, Roger; Puntervoll, Pål; Goldstone, Jared V.; Jakobsen, Kjetill Sigurd; Stegeman, John; Goksøyr, Anders; Karlsen, Odd Andre. 2018. Independent losses of a xenobiotic receptor across teleost evolution. Scientific Reports. 8: 1-13. doi: 10.1038/s41598-018-28498-4
  • Govasli Larsen, Morten Andreas; Diaz, Yuleima; Zegeye, Ephrem Debebe; Darbakk, Christine; Taxt, Arne Michael; Puntervoll, Pål. 2018. Purification and characterization of native and vaccine candidate mutant enterotoxigenic escherichia coli heat-stable toxins. Toxins. 10:274: 1-15. doi: 10.3390/toxins10070274
  • Zegeye, Ephrem Debebe; Govasli Larsen, Morten Andreas; Sommerfelt, Halvor; Puntervoll, Pål. 2018. Development of an enterotoxigenic Escherichia coli vaccine based on the heat-stable toxin. Human Vaccines & Immunotherapeutics. 15: 1379-1388. doi: 10.1080/21645515.2018.1496768
  • Schäuble, Sascha; Stavrum, Anne-Kristin; Bockwoldt, Mathias; Puntervoll, Pål; Heiland, Ines. 2017. SBMLmod: a Python-based web application and web service for efficient data integration and model simulation. BMC Bioinformatics. 18: 1-8. doi: 10.1186/s12859-017-1722-9
  • Bjerga, Gro Elin Kjæreng; Arsin, Hasan; Larsen, Øivind; Puntervoll, Pål; Kleivdal, Hans Torstein. 2016. A rapid solubility-optimized screening procedure for recombinant subtilisins in E. coli. Journal of Biotechnology. 222: 38-46. doi: 10.1016/j.jbiotec.2016.02.009
  • Taxt, Arne Michael; Diaz, Yuleima; Aasland, Rein; Clements, John D.; Nataro, James Paul; Sommerfelt, Halvor; Puntervoll, Pål. 2016. Towards rational design of a toxoid vaccine against the heatstable toxin of Escherichia coli. Infection and Immunity. 84: 1239-1249. doi: 10.1128/IAI.01225-15
  • Taxt, Arne Michael; Diaz, Yuleima; Bacle, Amélie; Grauffel, Cédric; Reuter, Nathalie; Aasland, Rein; Sommerfelt, Halvor; Puntervoll, Pål. 2014. Characterization of immunological cross-reactivity between enterotoxigenic Escherichia coli heat-stable toxin and human guanylin and uroguanylin. Infection and Immunity. 82: 2913-2922. doi: 10.1128/IAI.01749-14
  • Subramanian, Sattanathan; Sztromwasser, Pawel Szymon; Puntervoll, Pål; Petersen, Kjell. 2013. Pipelined data-flow delegated orchestration for data-intensive eScience workflows. International Journal of Web Information Systems. 9: 204-218. doi: 10.1108/IJWIS-05-2013-0012

More information in national current research information system (CRIStin)